FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580505
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTGG4151.7205163E-414.029311
CTCAAGG3350.009512713.0346961
CTCCTCG5300.001350115610.9852161
CTCATAA6450.0068657019.026611
CTTTGAT9250.0017924367.8677891
CATCAAT9600.00258172847.58094261
TATATTA8650.0092261367.51674035
CTGAAAT10600.00677898346.865761
CAAAGAA15200.00547478865.7455571
CCTTTTT16700.00271851525.6238023
Produced by FastQC (version 0.11.5)