FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410157
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT8556.996846E-59.4801671
CACACGA8003.757682E-49.2108441
CTTAATG16155.0260496E-88.2127661
CTCCAAT21601.672015E-87.16398951
CCCAGTT19952.3002212E-77.0177861
CTCAAAT21458.088464E-76.5270321
CTCAAAA33153.092282E-116.4462021
CTCAAAC18402.5035208E-56.40754371
CTTCGAA12750.0050229576.3572891
CGAAAGG17458.3093706E-56.3341051
CTCCTAT14050.00198683896.2935311
CTCGATT14150.0021517326.24905351
CAACAGT20501.8430866E-56.1106091
CTTACAT17054.2121066E-46.0505251
CTTCAAA43200.05.9699911
CTCTTGT16250.00155761315.89494041
CTCTTAT17606.3275814E-45.86144641
CATTAAT21403.5613517E-55.85362051
CTTGAAT20301.04536964E-45.80782271
CTCCATT30605.6450517E-85.7793531
Produced by FastQC (version 0.11.5)