FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3455240
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCTT16458.830102E-87.94377851
CTCACAG15753.1187228E-67.3749641
CTTCGTT33400.07.1727881
CTTTAAA46350.06.8916541
CTCAAGA20153.5497214E-76.8454031
CTTCTAT39600.06.7830821
CTCAGCT16253.8091675E-56.70128971
CTTCAAT52700.06.47452261
CTTAGAA13500.00149895796.45309451
CTCCATT38900.06.34526631
CCCCCGT12650.00540573246.31280951
CTCAGAA22101.7468574E-66.24139741
CTCCTAA13050.0074687476.11931321
CTTTCAT62950.06.1122431
CTATAAG19155.5786666E-56.06557131
CATAGAG21658.300909E-66.0358041
CTCTTTT69000.05.9971691
CTCCAAT29252.987872E-85.9567021
CCCCTTT29653.9810402E-85.8763421
CCCCATC29001.570861E-75.75771761
Produced by FastQC (version 0.11.5)