FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856383
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAAT9209.426003E-911.92124751
CTCAAAT6957.730727E-611.5724721
CTTAAAA10555.982656E-911.0888311
CCTCAAT6706.77662E-510.9129831
CTCAAGA4900.00912023310.4452831
CCCACGA6355.006509E-410.3630371
CTATTAT8400.00638053577.83396241
TCCTTAT9550.00267096147.55563553
CTTTCCT10950.0088798856.67734961
CAAAGAT12300.00373604226.53891661
CCCTTTT12750.00543589846.30813171
CTTCTTT24801.0872787E-55.60170461
Produced by FastQC (version 0.11.5)