FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1351628
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGAC8300.0065501847.8117381
CACGCAA8650.0094763947.49565651
CTTATGA16153.8822887E-56.69116831
CACCGAT14250.00299641586.066661
CTATAAT15500.00762339735.5774131
GTTTTAA15550.0078988975.5594791
CTTTGTT22401.00185884E-45.46744161
CAATTCT17200.00423808445.4180114
CTTTCAA23954.7263777E-55.4143991
CTTTAAT18700.0018167245.3934881
CTTTGAA23001.4911551E-45.3248131
CTTGATT20950.00134172845.15810871
Produced by FastQC (version 0.11.5)