FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298800
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT5606.6648136E-714.401551
CACACGT7108.9324567E-712.1525017
ACACGTC8204.0846862E-711.3991448
CACGTCT9051.4589605E-610.3269349
GCACACG10601.0436831E-48.1398826
CTTAACT9200.00158426637.9692381
CACCCAT16203.178672E-56.78860951
AGCACAC14354.672501E-46.5137975
CTCAAGG14755.149791E-46.46183631
CTCAGAT12550.0044886586.42619041
CTCTTCG20001.3611003E-56.2319441
AGAGCAC14050.0026162056.14112143
CTCCTAT14350.00266475356.13103771
CTCCCAT19205.0162937E-56.1097491
CTCAGAA17104.6650987E-46.002561
CCACAAT22151.0228263E-55.958041
CTCCAAG14850.00390680975.92460541
CTCCAAT18602.1360678E-45.91266061
ACCCATT14700.0043335155.86970232
CCCAAAT18852.562052E-45.83424331
Produced by FastQC (version 0.11.5)