FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100803
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTGA3254.1650093E-415.7508791
CCCCCAT4200.00288872112.1881811
CTCTCAC4300.003445222711.9047361
CCCCATT7251.2349054E-511.0954481
CTCACAA5952.741324E-411.0615421
CCCACAC6254.3202523E-410.5305881
CCCAAAT6850.00100338559.60821
CTTAGAA6850.00100338559.60821
CCCTCCT6100.00417320529.59071
CTCTCCT8901.176078E-49.0384261
CCCAGCT7600.00258550938.6600231
CTCCAAT7650.00274377888.6034211
CTCTATT7650.00274377888.6034211
CTTCTGA14609.800715E-88.515031
CTTCAAT16004.8643415E-88.2270221
CTCCCTT8950.00123663978.1708481
CTTCCGA9300.0018056017.86334231
CCTCCTT14004.2134106E-67.8352591
CTTGCAT10506.999228E-47.66114231
CTTTAAG9900.00333384547.38677641
Produced by FastQC (version 0.11.5)