FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498918
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATTT16751.4370016E-109.1419161
CCTTAAT8350.0062033567.8593891
CTCCAAG10557.5996213E-47.602791
CCCATGA10759.284715E-47.46134231
ATGATCG9950.0039720347.2557973
CCCAGAT12858.141608E-46.80943161
CAACAAT20404.0626583E-76.79135131
CTCCAAT13009.290041E-46.7308611
CAACAAA27409.677024E-106.6530721
CCCAGAA13300.00120336576.5790371
CTCTGTT12250.00368845846.5477091
CTCCCTT12300.0038490476.52109241
CTCAAAT12750.005599396.29093551
AGTTTTA13900.00221457226.2326774
GAGATTG15650.00126849155.99644335
CTTTTAT29252.7110218E-85.9829881
GTTCAAT14800.0039962095.91224341
CTACAAC14850.0041491155.8923371
CTCAAAA20051.0158953E-45.8188661
CAAAGAT25102.269393E-65.81017261
Produced by FastQC (version 0.11.5)