FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373104
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT8305.791748E-48.8204961
CTCTAAC7800.00324109528.4473211
CTCAAAG22851.8189894E-128.3302541
CTCGCTT9853.489086E-48.1757491
CTCCTAA9500.00220403037.70632741
CTCACAG12154.1038587E-47.23062851
CATCGAT19109.29258E-76.89938261
CCCAAAA20453.9613587E-76.80191761
CCCAGAT18403.8113812E-66.76397751
CTCTAAA12600.00475070956.3913591
CATTGAT40800.06.28027871
CTCCCCG14200.00240781076.18677041
CTCCAAT20652.2777933E-56.0269831
CTTTATT50800.05.90869051
CCTCAAT20251.1077827E-45.7845031
CTTATAT33059.218638E-95.75934361
TTCGATA27654.1672138E-75.74794672
CTCTCAT15300.00553266245.74196961
CTCCGAT19403.86083E-45.6605761
CTTCAAA45303.6379788E-125.65641071
Produced by FastQC (version 0.11.5)