FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722279
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT3000.004434302414.6277331
AGTTCCC4550.005753336511.113397
TCTACTT5350.001554595510.80183
CTCAATA6204.0030037E-410.6169031
CTCCACA6204.0030037E-410.6169031
ATTGAGG6050.0043118529.5520046
CCTCAAA7750.00307971528.4935221
CCCATTT7850.00345813588.38532451
CTTCTCT10205.118352E-47.88750361
CTTTAAT13151.5404712E-57.78662631
CTTCTAT9700.00272412377.5400681
AAATAGT9900.00375460167.296676
AGATTTA11953.943881E-47.2539284
CTTCAAT11400.00167356197.0572391
ACCCTTA2300.00210720026.906759310-14
ATCAATT12808.6541194E-46.7717532
CTTCCAA10900.0084683316.70996951
AGATTTT19501.6213744E-66.66803364
CTTCAAA13250.00111189346.6238791
CTTCATT13500.00137389866.50121451
Produced by FastQC (version 0.11.5)