FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316863
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4756.561095E-918.1493827
CACGTCT5504.0057785E-815.6744669
ACACGTC5304.4958506E-714.91045958
GAGCACA8405.629481E-711.118724
CGATTCA6200.00552393039.2701984
AGAGCAC9101.81593E-48.6844443
AGCACAC8407.86766E-48.5525365
GCACACG9954.7601323E-47.94225456
AAACATA13100.0086461396.0327054
GGCCGAT13250.0095285145.97621061
CTTCAAT17457.633132E-45.77539441
CAAATCC15800.00970220755.45650863
ATAATCT15850.0098290445.4500571