FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309379
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGTGT7304.0742953E-49.1360036
CCCAAGC7454.687211E-49.0088241
CGGTTAC10503.2367643E-77.9903526145
TCACTCG5501.6487376E-57.627155145
CCTTGAG14352.500541E-67.48328731
AGTCACT6102.8118484E-76.5293784130-134
GTTACAT8259.487485E-86.201725140-144
GTCAGCT13000.00236859826.1952991
TCGGTTA8808.172719E-96.0456305135-139
ATGCGGA14100.0062821025.6759858
CACTCGG7306.90237E-65.456056130-134
GGTTACA9752.395771E-65.2476134140-144
TCAAGAT20504.983532E-45.2054923
CAAAAAT19750.00156999475.0973981
CCAGCAC18700.004405395.0247081
Produced by FastQC (version 0.11.5)