FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296087
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGA9601.767512E-811.4268541
CTCAACT6506.200208E-410.1259511
CTCCAAG8456.675796E-59.5201251
CTTCAGA12558.843017E-89.3235841
CAACACG7450.00215754598.83472251
CTCAGGA8709.340287E-48.4059611
CTCTGCA13101.6327322E-68.3738781
CTCAACG8759.886406E-48.3579271
CTCCGTG8300.0057240937.92996171
CTTTGAA21452.6011548E-107.84164521
CACATCT9400.0020058097.7799861
CTTTCGT10356.0005387E-47.7724691
CTCCCAG9550.0023433197.65778731
CTCTGAT9550.0023433197.65778731
CTCCAAT10557.363259E-47.6251241
CTCCTGA13502.1931939E-57.5840461
CTCCAAC9700.0027302087.53936771
CTTCAAT21552.3464963E-97.46589851
CTTTCGA10000.0036773497.3131871
CTTCAAG17351.5372934E-67.16565851
Produced by FastQC (version 0.11.5)