FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036450
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAG6405.7417975E-410.2104421
CTTAAAT10105.21288E-69.345531
CTCCCGA6400.0065725099.0759481
CTCTTGT8901.2711578E-48.9739711
CTTTAAA15553.023706E-77.9378061
GTCAAAC8350.0064416357.8259671
CTCCATT12608.3805266E-57.49125771
CTCATAA10709.238252E-47.46433071
ATCAGCT13702.930296E-57.4197531
CTTTATT23351.3533281E-86.84097151
CTTCAAA18404.3235323E-66.7083091
CAAAAGT13100.00106274526.6510761
CTTCAAG15451.421546E-46.57932761
AATTATG14200.00277690736.10770855
AATCCGT13250.00913879656.0001517
CTTCAAT17607.623731E-45.77560331
AAATTTA17809.334457E-45.6845154
GATTTTG22952.2714581E-55.6685925
CTTCATT18950.00194426435.36414861
TAAATTT18950.00206001785.3395453