FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966611
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAT17101.4600464E-67.189831
CCTCAAT18205.108177E-77.152651
TTCGATT17351.9421022E-67.06081772
CTTCGTT22557.3287083E-97.05573031
CTTAAAG15602.2708478E-56.9539651
CTTCGAG21953.2538082E-86.91911551
CCCACAT14756.0773856E-46.3740751
AATTGGT13100.0084067626.0494597
CTCAAAT17055.353049E-45.93840031
CTTCAAT33502.7066562E-95.82887551
CTTCAGA28551.234148E-75.8262291
CTTTAAT18853.0843265E-45.75500541
CTCTTGA22701.8912167E-55.73472361
CTCTGAT17809.273594E-45.68818761
CCCAGAA19504.879437E-45.56317231
CTTCGAC21102.3796191E-45.48407461
CTCATCT22551.0463383E-45.45215561
TTCGAGA18600.00169344845.42401272
CTTCATT35104.7008143E-85.3571291
CTTTGAT32653.224468E-75.3161091
Produced by FastQC (version 0.11.5)