FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470235
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2453.2214302E-928.2556068
CACACGT2503.9963197E-927.6904957
CACGTCT2753.6379788E-1027.6904939
AGCACAC3603.274181E-1023.0778665
AGAGCAC3053.304558E-822.7019543
GAGCACA3505.9353624E-921.7614424
GCACACG3358.925417E-820.6667446
AAGAGCA5451.4239129E-512.7088182
GAAGAGC6203.8261223E-612.3226951
TCACTCG1700.0012640618.15362725-29
CCAGTCA2550.0094432928.1442638
CAGTCAC2805.38348E-47.6556225145
CTCGGTT2704.472925E-57.18727125-29
CTCGTAT2355.239791E-47.07804235-39
TCTCGTA2458.428841E-46.78914235-39
CAAAAAA12550.007845696.0877061
ACATCTC4150.00195984985.782426140-144
TGAACTC3250.00327126455.5385715-19
Produced by FastQC (version 0.11.5)