FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209935
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGAG2050.00875630917.8108221
CCCACGT3650.001015423714.0046721
CCGCGTT5852.3744015E-411.2345171
CTCCTAT6656.366196E-510.9811081
CAAGACT7351.7543072E-49.9352871
CTCCAAC8355.954363E-59.6199751
CCCCAAT8456.783092E-59.506131
CTCAGAT7000.0012395449.3888471
CTCATGG8054.3734978E-49.0713491
CTCCGCT8406.6868385E-48.6933771
CTCCAAG9251.816616E-48.6839781
CTTACAT7750.00312676568.4802491
CCCTCTT8709.4764127E-48.3936041
CTTCAAG17653.5688572E-98.2747151
CTCCTGA8900.00118698.2049851
CTCTGAT9250.00173689637.8945261
CCCATTT13051.4239971E-57.8340311
TCACGAA9300.00205972867.7589452
CTCAAAT10406.417452E-47.72373061
CTACAAT10507.108756E-47.65017181
Produced by FastQC (version 0.11.5)