FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910923
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCTGCACTTATCTCGTATGCCGT11370.12481845337092158TruSeq Adapter, Index 7 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4650.048.9048469
CACACGT5550.040.974337
ACACGTC6300.037.1902668
GCACACG8300.028.2287586
AGCACAC8650.027.0865525
AGAGCAC8650.026.2913323
GAGCACA9800.024.6139074
AAGAGCA16250.013.5769482
GAAGAGC16150.013.2676281
CCCTATC3700.001741676213.0372867
CAGTCAC5150.010.17100920-24
GCACTTA5700.09.18939430-34
AGTCACC5750.09.10968720-24
CCAGTCA5600.08.86139620-24
GTTTGGT6400.0098361418.6400071
ACGTCTG6450.08.11943310-14
AAAAAAA28100.07.700924460-64
CGTCTGA7350.07.68773410-14
ACTCCAG7400.07.07722915-19
CACTTAT7250.07.0346430-34
Produced by FastQC (version 0.11.5)