FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526692
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2801.03587045E-519.476349
AGCACAC3554.1034145E-617.2867475
AGAGCAC3104.944477E-415.3969763
CACACGT3257.092358E-414.6821647
GAGCACA3409.958151E-414.038424
GCACACG4152.9922154E-413.1419116
ACACGTC4200.004878609411.3611988
TCCATTG5500.00315239269.9199372
AATCCAC5650.0039443129.6547415
CAAAGAT6300.0093648488.6908051
TTGAAAA13650.00342532015.99272067
Produced by FastQC (version 0.11.5)