FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079437
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAC7701.6256126E-712.2360351
CCAAGTC5900.00352292049.7893288
CTCCGCT7652.8303146E-49.4738541
CTCAGAA8701.0125327E-49.1635031
CTATGAC7300.00194617178.9352711
CTCCGTT8407.207182E-48.6279741
CTTGAAT13101.8565897E-68.2986621
CTAAAAT14256.1522624E-67.6289451
ATGTATG11703.117443E-47.40474846
CTTATGG10100.00442298977.17574071
CTTGAAG13702.4048903E-46.87718771
CATTAAT13250.00123452196.56377171
CTTCAAC13300.00128827956.53909541
CTTCCGT13750.00187539986.32508951
CATCTAT12700.0057231086.27736041
CTTGTAT13100.0078952836.08568571
CTTGGAA13200.0085414576.03958131
CTTTTAA21951.0615102E-55.94327831
CACCTTT15300.0061782365.68431231
CTTTCTT33401.4611942E-85.64176461
Produced by FastQC (version 0.11.5)