FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1224434
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT5109.560091E-411.4500231
CTCTAAT6909.3001654E-510.5788251
CTTAGAA10951.2765083E-47.99933051
CTCAAAC10305.813007E-47.79546451
CCTAAAG8650.0084269167.5947411
TTTTGGC15452.6188336E-67.4601287
CTCAAAG13903.3111035E-57.35190341
CTCTTAT13400.00129386256.5367671
CTTCGTT12400.00414199346.4752651
CTTCCAC14705.2073394E-46.4552421
GATTCAT18503.7671998E-56.23021465
CTTTAAT24752.6497946E-76.19342141
CTTCTAT18904.2452128E-56.17937761
TAATTTA21851.0882293E-55.93438674
CTCAAAA17305.7221006E-45.90702061
CTTAAAG15050.0047569225.82011131
GTAAAGA17357.0285745E-45.81277282
AAGGTTT14900.00492810645.8016263
CTTTGAT29251.664921E-75.7396911
GTGGGGG3250.0074558785.589449140-144
Produced by FastQC (version 0.11.5)