FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340574
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT9552.6627853E-59.1455361
CTTTCGT10701.0514104E-58.8428221
CTCTTGT8709.794106E-48.3658881
CTATTCT9350.00199501177.78430271
CTTCGAT14557.78617E-67.5034261
CTAAAGA10050.00404506137.2421121
CTTCTTA10350.00538517167.0321961
CTTTCAT19752.3871326E-77.00193071
CTTAAAT10500.0061918256.9317361
CTATTAC10750.0077749936.77053261
AAAGATC10650.0079515246.7548262
CTTGTGC10850.0085020396.7081321
CATTGAT14906.354651E-46.35021451
GTTTCAA12950.00670833566.1823591
GGTGAAA16600.0081075026.07008145
CAAACTT13300.0088417626.01966571
CTTTCGC13400.009552845.9747431
CTTGTTT15900.00138971755.95082951
CTTTTAA19053.2612213E-45.73096281
CCACCAT16050.0099954695.4417371
Produced by FastQC (version 0.11.5)