FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489304
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1551.1314114E-940.3037227
CACGTCT2209.895302E-1031.5508949
ACACGTC2102.1944288E-829.7479868
GAGCACA2305.30963E-827.1612054
GCACACG2708.822099E-925.7081346
AGAGCAC2751.07247615E-825.2407153
AGCACAC2955.877373E-721.1765335
GAAGAGC5404.660069E-815.4770731
AGTATAG2900.005007461614.3610965
CCTATAT4300.004953102711.3378551
AAGAGCA6851.1660226E-511.1498662
CTTTGAT9000.00210821167.73853641
TTTACCA8150.007743457.6651257
CAGTCAC2600.00162396636.407258520-24
GATCTCG2650.00201022536.287009235-39
TTTATTT15703.1242904E-46.1896023
TGAACTC3600.00179687995.9738755145
AGTCACC2900.0055066445.74443920-24
CTTTTTT15950.00243066465.67654371
CCAGTCA3400.00540068845.307973420-24
Produced by FastQC (version 0.11.5)