FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548333
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATT2501.1354886E-418.6694761
CTTAAGG1850.00817915418.020731
CCCACAT3508.502867E-515.2403891
CTCATAT4150.00526176111.2466721
TCTCTCC4400.00822263610.591215
ATCCAGT6158.6968276E-49.75756551
CAATTTC6150.0096424398.6599454
TCAAGAT6150.0096424398.6599453
AAGGAAT9550.0058486246.9710293
CTTTTGC9700.00670052556.87388661
CTTTTTC18200.00339899845.1289771
Produced by FastQC (version 0.11.5)