FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399737
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT2500.00140114117.3818951
TTAAACT3305.155778E-415.3108893
CCGGCCT2900.003863389414.9319057
ATCCGAT4600.006113433711.0211281
CTATTAT5750.002766349610.076461
CTTCAAT7452.1760681E-49.7214171
CTTTAAA6550.0080963678.8457471
CTTTTTC17550.0049340925.34599075
Produced by FastQC (version 0.11.5)