FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7729423
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACT13705.4227712E-88.8354161
CTCGAAT27700.07.96857261
CCCAAAT49450.07.919451
CCCAGAG30851.8189894E-126.9241221
CTCAAAT67300.06.8769661
CCCCTGA28355.18412E-106.5300861
CTCCGAT65050.06.34861951
CCCCAAT38300.06.3208981
CTCACTA16908.5835134E-56.3197981
CCTCGAT61400.06.2621581
CCCAGAA63050.06.211211
CTTCGAG48500.06.16603761
CTTTGAT132150.06.08849431
CCCAGCT63400.06.06461331
CTCGCAA28202.08056E-86.05983451
CCTCAAT60000.06.052261
CTTCGAT76350.05.96855971
CTCTAAT28451.5810656E-75.756311
CTCAGTT31052.6235284E-85.73293541
CCCCCAT31152.8143404E-85.7145311
Produced by FastQC (version 0.11.5)