FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476128
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGCT3100.005827639714.0362751
CCTCAAT4400.004348338611.5373941
CCCATTT5150.001093252711.2653611
CTCTTTT11603.1973013E-68.7525051
CTTCAAT7700.00313229678.4764521
ATTTGCA8450.00740865257.7036
TTAAAAT8600.0086707057.56864552
TCCGGAT10250.0051976497.05586432
CAAAGAT10650.00733541466.80946061
ATTAAAA10750.0080286956.74611661
CTTTTTT18903.059258E-45.75561571
Produced by FastQC (version 0.11.5)