FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences750144
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC2750.002596175315.8527111
CCCAAGA4401.724442E-514.8619171
CACGTCT2950.00439806514.6459539
CTCCCGA4552.3666265E-514.3719631
GAGCACA3050.005480080414.1685914
CCCAGAG3150.0064030913.8396681
CTCAGAT3800.001428496213.3844181
CTTCTAA4604.1713892E-412.6362181
CTCATGG5250.001265237711.0717351
TAAGAAC5700.002699031310.1085854
ATTCGAT5950.00358123829.7691771
CTCAAAG7502.2592717E-49.6877691
CTCTCTT6850.00106365699.5463411
CCCATTT8400.0067488717.78481341
TTTCGCC8600.0090147847.53685867
CTTCCCG9850.00337659267.37647341
CTTTATT15101.3422441E-57.2177081
CTTTCAT12657.077764E-46.8924831
CTTTTAA11600.00215612686.8900071
CTCTTCC10800.0082573426.7276171
Produced by FastQC (version 0.11.5)