FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748869
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGA4750.007353741710.7525981
CTCTTGC7201.4349497E-410.1338971
GTCAGGT6400.00630977849.1205071
CTTCGGT8054.4047245E-49.0638571
CTTTGGA8356.3457614E-48.738211
CTTTAAT8558.030484E-48.5338081
CTTCTAT8950.00126339138.1524091
CTGTAAT8400.0064995097.81757741
CTTCAAG8700.0088963567.5480051
CTTCAAT10658.333275E-47.53619341
CTTCAAA13702.2114001E-46.9235971
CTTTTAT13250.0011425936.6080651
CAAAAGA14150.0023972146.1877641
CTTCTTT20156.711245E-45.4315671
AAAGAAT19000.00219148055.31276132
Produced by FastQC (version 0.11.5)