FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509149
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTTA3750.003167637615.383676145
CAATCGC3650.00133567613.5028514
CCCAGAA4100.003143764812.0492511
CTATGAT4706.381387E-412.0126271
CCTCGAT6254.7789406E-511.2918691
CTTTCGC4450.00579512611.1015571
CTCCGAA5350.001885787910.5531481
CTTTGAT7503.0223874E-49.4098911
CTTTTCT9955.74284E-47.80217081
Produced by FastQC (version 0.11.5)