FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620794
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2508.3868144E-825.9123887
CACGTCT2506.3105435E-520.154089
ACACGTC2952.2444359E-417.079738
GCACACG3757.976927E-515.355496
AGCACAC3559.169904E-414.1930145
ATATTAC5953.1706438E-410.8875586
AGAGCAC4700.007517779710.7202553
TTCAGGG5750.002898027710.0217152
CCTTGGT6450.0071860618.9767781
GTCAGAA6650.0092288718.70681
CGAAAGA6700.0098118948.6418231
AAATGAA12100.00370469046.5435335
GGTCCTC3150.00141918545.94111290-94
Produced by FastQC (version 0.11.5)