FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009066
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGG18354.359941E-66.7062531
CTCGAAG12900.00682575076.1726271
GTTACAT18802.9402022E-45.7756451
CTTGGTT15200.00583205745.71484851
TATAGCT15500.0075313935.58412367
CTCCAAC19755.7256594E-45.4978291
GGTCAAA25602.1991204E-55.3725531
GTTTGAC21603.2810104E-45.362081
GTTGAAT20308.278665E-45.34887271
TTTACCC19150.00241626735.2730847
Produced by FastQC (version 0.11.5)