FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515815
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT2750.002702931615.7559053
CCTCAAA4703.0588497E-513.9842441
CTCCGAT3300.008417455513.2779681
CTCAAAG4906.7956E-411.9230751
CACCTGT4950.009911614510.3273091
CATAAAT7251.5229873E-410.0729421
CTTCCAA6150.0045079069.4996851
CTATTTT9300.00182606387.85256241
CCTCTTT8700.0088167767.5547061
AGATTTT10050.004355457.18552834
CTTCCGG12405.307494E-47.0673061
TGAATAT10750.0083663256.71763373
CTTCTTT14450.00299714046.06467721
Produced by FastQC (version 0.11.5)