FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589740
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAT3254.5368692E-415.572761
AATCGAA4503.6099303E-412.8537051
CCTCAAC6604.917287E-612.050351
CTCAAAT4300.003747552211.770111
CTCCTCT4500.005261431411.2469931
CTTCCAG10406.757E-810.4281871
CTTGAAT6305.1562075E-410.3288721
CGAAATA5750.002868798610.0338294
CCTTTAT7600.00286204958.562091
CTTCAAT8800.00116884448.2161471
Produced by FastQC (version 0.11.5)