FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026850
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAG8859.1906804E-710.7032591
GTCGAAT4900.00935913110.4092141
TGCACGG7753.0653602E-510.21913057
CTCAAGA7200.00163468839.1080621
CTCTATT7250.00174101889.0452481
CCCGGAT12759.2877715E-57.4293221
CTTCGGT10250.00484517737.10873131
TTCAGTA10500.00688712766.85565669
CTTTTAT20855.991351E-76.63993071
CTTATAT13450.00137624216.5009221
CAATTAT13400.0015096026.44793373
CTTTGAT15901.9718433E-46.41574241
CTTGTTT17308.5902146E-56.31773141
CTTCAAA18603.4466444E-56.26791331
AAGATTT23606.1151113E-65.79676533
CTTCAAT18009.702011E-45.66723871
CAAAGAT17050.00314859285.55565071
CTCCTTT18900.00179876525.39737031
CATCAAT19200.00219278765.3130371
Produced by FastQC (version 0.11.5)