FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1354460
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT5150.022.3493429
ACACGTC4850.022.2485398
CACACGT5201.8189894E-1220.7510437
GCACACG8703.7107384E-1013.2297836
AGCACAC10658.962161E-910.8074285
GAGCACA13704.5676643E-88.9274714
AGAGCAC12459.948471E-78.6680633
CACATAT9352.3026126E-48.4961021
GTTTAAT8609.4369904E-48.397311
GTATAAA9300.0020437097.76525551
TCCAAAC10750.00106466677.36588432
GGTAAAC12951.258238E-47.249571
GTTGTAT10650.0076608276.7809261
GGTGGAT14957.2737236E-46.27972751
GGTATTG18151.8772356E-45.9683361
AGTCACT4153.0907257E-55.90872420-24
GAAGAGC23501.9843355E-45.22419931
GTCACTC5354.850902E-55.122626320-24
AAGAGCA22506.314239E-45.1189182
Produced by FastQC (version 0.11.5)