FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230262
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACAGAACGAGATCTCGTATGCCGT13650.11095197608314326TruSeq Adapter, Index 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6450.033.1948287
CACGTCT7450.028.7391459
ACACGTC7900.026.2551678
AGAGCAC8600.024.8940963
GAGCACA9150.024.128914
AGCACAC9900.022.300965
GCACACG10500.021.0266196
AAGAGCA15050.013.78850652
GAAGAGC16650.013.3288291
CTTCGAT13305.4552336E-57.07894751
CTCGTAT7350.06.92950935-39
CGAACAA9950.0083104916.72390845
TCGTATG8455.456968E-126.3311706145
CTTCTGG14350.00104785666.09233431
AGTCACA7601.8189894E-126.00361620-24
CTTTCAT17503.0137112E-45.76428561
CGAAAAT16900.00128819915.54225063
AATTCCT14650.0092434555.4805457
TCTCGTA7358.112693E-105.470665535-39
GAGATCT8554.9112714E-115.32530330-34
Produced by FastQC (version 0.11.5)