FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973882
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAC6153.7560627E-410.6911261
CTCCGAT7853.3835505E-49.30651
CTCTGAA8356.2709325E-48.7492251
CTCAAAG10155.2110852E-58.6371661
CCCAGAT7650.00276813038.5948271
CCCATTT10255.8452504E-58.55291
CTCCGTT8608.4092346E-48.4948861
CACCGAA9150.00155310547.9842661
GTCAAGA8600.0079378797.6453981
CATCGAT8600.0079378797.6453981
CCATAAC8650.0083599947.6012061
AAGTTTA9750.00328954247.39672474
CTTTGTT14405.2155654E-57.10266971
CTTCTGT15351.2050903E-46.663091
CTTCCAA13450.00133578776.518011
CTTTGAT19858.53708E-55.88864761
TCAAGAA18602.665261E-45.8162722
CTTCAAA17150.0032704775.5377751
CTTCAAT15950.0089493795.4963781
CTTTTGT17700.0047466345.36569741