FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497735
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1751.593146E-423.9969717
AGAGCAC2503.0788106E-622.4061763
CACGTCT2003.932662E-420.9973479
ACACGTC2258.694578E-418.6643098
AGCACAC3053.5759283E-416.0667725
GAGCACA4051.1175396E-515.5567174
GCACACG3150.0081847513.3343296
AAGAGCA5752.9807643E-410.9595422
CTGAACT3000.007307985.59929310-14