FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685994
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAC4750.007643223310.6981081
CTCGAAT7151.4102599E-410.1530491
CTCAGAG6500.00747971248.9346831
CTCACCC7700.00311214928.4850491
CCCAAAT10357.062008E-58.416731
CTCCAAT14801.3758472E-78.3385341
CTTAAAC10155.271547E-47.8673631
CACACGG8600.0084071827.59707831
CCCCTTT13452.304368E-57.55628441
CTCAGAT8750.0098100387.4668421
CTTTAAT25954.1836756E-117.2734181
CTCCTCT12003.9188805E-47.25943041
CTTCTGA20104.443791E-87.22331331
CTTCAAT27652.5465852E-117.08877471
CTCTCAA10500.0063518346.9137431
CTTCCGT18404.345078E-66.70708231
CCCAGTT10850.00872074356.6907191
GTCTTCA16354.1451414E-56.6600281
CTCAAGG14203.6569848E-46.64595751
GGTTGTT15351.3112334E-46.6209791
Produced by FastQC (version 0.11.5)