FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701814
Sequences flagged as poor quality0
Sequence length101-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGT4701.0148324E-716.9939981
CTCAACG3203.9049285E-415.8835941
CCCATCT5705.437505E-815.2864661
CCCCCAT3350.00966013113.0049031
CCTGCGA4800.00823882110.5890621
CCCTGAT5950.00360021539.7627851
CTTATCT8450.00715806247.7336851
CCATTCT8700.0096264427.48146963
CTTTCGG8750.0097760777.46853071
CTTTTAT16204.7096146E-67.1714281
CTCTTTC12707.4567395E-46.8608551
CTTTGAA13009.724575E-46.7025281
CTTTCCT12350.00418910576.4673511
CTTCCTT13100.00773459076.09708261
CATCTTT15350.0065488865.6537493
Produced by FastQC (version 0.11.5)