FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566362
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACAGAACGAGATCTCGTATGCCGT6840.1207708144261091TruSeq Adapter, Index 2 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2400.065.279347
CACGTCT3000.054.5972639
ACACGTC3050.051.367358
GCACACG3550.046.1426056
AGCACAC4650.035.2271545
GAGCACA4500.034.818724
AGAGCAC4700.033.3370743
GAAGAGC7750.020.2979681
AAGAGCA8550.018.3385962
AGTCACA3150.010.41768920-24
AGATCTC3450.09.91834335-39
CAGTCAC3351.8189894E-129.79573720-24
TCTCGTA3907.2759576E-128.77391835-39
ACTCCAG4001.0913936E-118.54565915-19
ACGTCTG4152.5465852E-118.2367810-14
CTCGTAT4253.6743586E-107.71588835-39
CTGAACT4704.0745363E-107.272901510-14
AACGAGA4901.3096724E-107.2712130-34
ACGAGAT4901.3096724E-107.2712130-34
GAACGAG5104.1836756E-117.26550830-34
Produced by FastQC (version 0.11.5)