FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509696
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCAAG3750.001578172313.2072471
CTTCAGT4400.005411056311.2042738
CCCGAAA4500.006180015411.0060381
CTTCGGG5250.001577861110.78142451
CACCTGT4650.00816004910.6018939
GTTTCAA6609.6410327E-49.648151
CTCATAA6150.0058508159.2036551