FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838117
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA13503.1079435E-78.5899551
GTCGAAT8300.0062072687.8590321
CTTGAAT22354.406502E-86.809991
CCCTCTT12450.0046550596.40365651
CCCCAAA14755.919538E-46.38786941
CACCAGT12500.004853756.3780421
CTCCAAC14250.00280227816.10338971
CTTCAGT13200.0085458486.03981261
CTAAAAT18652.5905724E-45.82930951
CAATCAC17250.00385409345.4620911
CTCCAAT19350.0025885365.2438681
CTTCCAT37403.3523975E-85.23235131
GTCAAAG19400.00267355595.23035341
CTTCAGG18450.0084823995.10683351
CCCGAAA21300.00154437115.1040671
CTTTTAT46101.3005774E-95.03099351
Produced by FastQC (version 0.11.5)