FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1150850
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTGT4755.1287044E-412.3312381
CTCAAGA8403.3507604E-812.2027881
CTCCTAC5751.9742119E-411.4600091
CCCCTTC7301.3152561E-511.0326571
AGATCTC5500.001952392510.5113196
CTCACAA5650.002190270510.366971
CTCTGAT8506.2314666E-610.3364781
CCCTTGT6506.1332807E-410.13771
CCTCTCT5950.0033637739.8442651
CCCACAG7602.3924097E-49.633781
GATTTGC13804.773028E-99.4259115
CTCATGC8054.2595356E-49.0952441
CTCCTGT11301.980712E-69.0710981
CCCGTAT7300.00177416079.0267191
CCTTTAT18308.185452E-118.8020111
CCCAGAA10851.1624368E-58.7725111
CCCACGG7700.00287931538.5577981
CTCCGCT8850.00109357578.2730761
ATCTAAT8900.00115629398.2265991
CTCCTTT22307.2759576E-128.2081531
Produced by FastQC (version 0.11.5)