FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1858130
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAG14155.2979885E-88.8467281
CTCAAGC11401.8412567E-47.75115781
CACAAAG22253.8999133E-97.28086331
CTCAAAT15501.236358E-46.65099331
CCCCTTT15951.7944188E-46.46334841
CACAAGG13200.0072523966.13633351
CAACAAA29001.175722E-75.84009651
AACCGTT18602.6292933E-45.822972
CTTCAAG24055.774831E-65.8173971
AATTTAC18300.00134531145.52371845
CAATAAG18850.00152567535.46898651
CTTAAAA23301.305278E-45.3725841
CTCTGTT17900.00494371355.34786561
ATTTACC19950.00397396975.06686936
TAATATA30101.1957663E-55.03741074
CTTCAAC21950.00186410735.0320731
Produced by FastQC (version 0.11.5)