FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145363
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTAAC5401.2578096E-412.0287345
TTGGTTA5900.0035379659.7843799
CACGAAT6100.00451196879.5004911
CCAATCT7400.0022877468.7777243
CCCATAT8306.42782E-48.7278611
CTTTAAT12701.1985339E-68.5560541
CTCAAGG8558.6339406E-48.4726611
CTCCGAT9452.4971642E-48.4323151
CTTCAAT15152.0679727E-78.1287211
CTCTTAT8650.0090178827.537241
CTCATGG10000.00403308737.24412541
CTTTGAA17852.7885362E-66.8991671
CTCCAAT10500.00647926246.8991661
CCCTTTC10500.00647926246.8991661
CTCACCG11600.00222776486.8694291
CTATAAA10550.0067842446.86646941
GTTGCAA10650.00771747166.7755688
CTATAAT10850.0088949336.6766131
CTTTGAT19551.6195718E-66.66978261
CTTCTTT32001.3278623E-106.3386091
Produced by FastQC (version 0.11.5)