FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998890
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGTC5050.001564829310.7940559
CTCAGAC5300.00213585289.147783145
GTCCGTT8500.00140987178.0629971
TCCACGG10109.829552E-47.42091277
CACTCAG5451.672368E-66.65827140-144
AGTCACT4351.7088099E-46.4881988140-144
GTCACTC5103.0315887E-56.324631140-144
CAATATT12000.00602862566.2459354
ACTCAGA5206.717325E-45.432988135-139
CAAAAAT17400.0082113135.1204661
Produced by FastQC (version 0.11.5)