FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400623
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCATT13051.9252002E-78.8629481
GTTTGCT12100.00354969556.5716741
GTCATTT13250.00127184946.5468761
GTCGGAA12250.0040380276.49120471
GTTTTGG12750.00612677076.23664761
GTGCCGA15309.503255E-46.14215331
GCAGGTT15550.0077738655.5675747
CTTCCAA17100.00358352655.495611
CTTTGGC17150.0037092965.4795891
CTTCAAT21703.5664026E-45.3300221
AACTTCA19200.00248195745.26124435
Produced by FastQC (version 0.11.5)