FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005014203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005014203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513550
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAG450.009950994547.9419759
TGAGCAT4202.10692E-413.6977068
CAGAGAG4500.0050980511.2942241
CATTGTA5802.5225314E-411.1589077
CCCAGCT4700.007045942410.8136181
CTTCGAT6204.2780826E-410.539541
AAAAGGT7800.00379867138.2976493
AATATAG8200.00595773857.89288575
CTTCAAT8600.0083753177.5982731
CATCAAT10800.00101770847.3950271
CTTCAAA11150.00142792837.16289661
CTTCTTT18602.4237923E-45.85531
Produced by FastQC (version 0.11.5)